Description

Single-Cell Western: Precise Protein Quantification at the Single-Cell Level

Revolutionize your cellular analysis with Single-Cell Western (SCW), a powerful technique offering unprecedented sensitivity and precision in quantifying protein expression at the single-cell level. Unlike traditional Western blotting which analyzes protein expression in bulk populations, SCW provides a detailed, single-cell resolution view, revealing cellular heterogeneity and uncovering hidden subpopulations within your samples.

Unmatched Advantages:

• Single-Cell Resolution: Analyze protein expression in individual cells, overcoming the limitations of bulk analysis and revealing hidden heterogeneity within your cell population. Identify rare cell types or cells exhibiting unique protein profiles.
• High Sensitivity and Precision: Detect even low-abundance proteins with exceptional sensitivity, enabling the identification of subtle changes in protein expression across individual cells. Precise quantification minimizes experimental variability for robust data analysis.
• High-Throughput Capability: Analyze hundreds or thousands of individual cells in a single experiment, enabling high-throughput screening and the study of diverse cell populations.
• Versatile Applications: Compatible with various cell types and sample preparation methods, making it applicable to a wide range of research areas, including immunology, oncology, neuroscience, and stem cell biology.
• Reduced Sample Consumption: Requires fewer cells compared to traditional techniques, conserving valuable samples and reducing experimental costs.
• Automated Data Analysis: Streamlined data acquisition and analysis software enables efficient processing of large datasets and minimizes manual intervention.

How it Works:

SCW utilizes a microplate-based format, allowing for the simultaneous analysis of numerous individual cells. Cells are lysed directly in the wells of a specialized plate, followed by antibody incubation and detection using chemiluminescence or fluorescence. The resulting signal intensity directly correlates with the protein expression level in each individual cell. This allows for precise quantification and statistical analysis of protein expression patterns within the cell population.

Applications:

• Identifying and Characterizing Rare Cell Populations: Detect and characterize rare cells expressing specific proteins within a heterogeneous population.
• Studying Cellular Heterogeneity: Reveal the extent of protein expression variability within a cell population, providing a deeper understanding of cellular function and behavior.
• Analyzing Drug Response at the Single-Cell Level: Assess the heterogeneity of drug response within a cell population, identifying potential therapeutic targets or resistance mechanisms.
• Investigating Signaling Pathways: Study the dynamics of intracellular signaling pathways by analyzing protein expression changes in individual cells under various stimuli.
• Monitoring Cell Differentiation and Development: Track protein expression changes during cellular differentiation or development, providing insights into cellular lineage and fate decisions.

Our Single-Cell Western System includes:

• Specialized microplates: Optimized for single-cell lysis and detection.
• Comprehensive reagent kits: Including optimized lysis buffers, antibodies, and detection reagents.
• Automated data acquisition and analysis software: Streamlining data processing and analysis for efficient workflow.
• Technical support and training: Expert guidance to ensure successful implementation and data interpretation.

Enhance your research with Single-Cell Western – the gold standard for single-cell protein quantification. Contact us today to learn more and discuss your specific research needs.